Partial Purification and Characterization of a DNA Helicase from Chloroplasts of Glycine Max

Authors

Gordon C. Cannon, University of Southern MississippiFollow
Sabine Heinhorst, University of Southern MississippiFollow

Document Type

Article

Publication Date

9-1-1990

Department

Chemistry and Biochemistry

School

Mathematics and Natural Sciences

Abstract

A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg²⁺ or Mn²⁺. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.

Publication Title

Plant Molecular Biology

Volume

15

Issue

3

First Page

457

Last Page

464

Recommended Citation

Cannon, G. C., Heinhorst, S. (1990). Partial Purification and Characterization of a DNA Helicase from Chloroplasts of Glycine Max. Plant Molecular Biology, 15(3), 457-464.
Available at: https://aquila.usm.edu/fac_pubs/7472