A Filter-Based Propidium Monoazide Technique to Distinguish Live from Membrane-Compromised Microorganisms Using Quantitative PCR

Authors

Kristin N. Hellein, University of West FloridaFollow
Elizabeth M. Kennedy, University of West FloridaFollow
Valerie J. Harwood, University of South FloridaFollow
Katrina V. Gordon, University of South Florida
Shiao Y. Wang, University of Southern MississippiFollow
Joe Eugene Lepo, University of West FloridaFollow

Document Type

Article

Publication Date

4-1-2012

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. (C) 2012 Elsevier B.V. All rights reserved.

Publication Title

Journal of Microbiological Methods

Volume

89

Issue

1

First Page

76

Last Page

78

Recommended Citation

Hellein, K. N., Kennedy, E. M., Harwood, V. J., Gordon, K. V., Wang, S. Y., Lepo, J. E. (2012). A Filter-Based Propidium Monoazide Technique to Distinguish Live from Membrane-Compromised Microorganisms Using Quantitative PCR. Journal of Microbiological Methods, 89(1), 76-78.
Available at: https://aquila.usm.edu/fac_pubs/8315