A Filter-Based Propidium Monoazide Technique to Distinguish Live from Membrane-Compromised Microorganisms Using Quantitative PCR
Biological, Environmental, and Earth Sciences
Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. (C) 2012 Elsevier B.V. All rights reserved.
Journal of Microbiological Methods
Hellein, K. N.,
Kennedy, E. M.,
Harwood, V. J.,
Gordon, K. V.,
Wang, S. Y.,
Lepo, J. E.
(2012). A Filter-Based Propidium Monoazide Technique to Distinguish Live from Membrane-Compromised Microorganisms Using Quantitative PCR. Journal of Microbiological Methods, 89(1), 76-78.
Available at: https://aquila.usm.edu/fac_pubs/8315