Linking Probe Thermodynamics to Microarray Quantification
Document Type
Article
Publication Date
12-1-2010
Department
Coastal Sciences, Gulf Coast Research Laboratory
Abstract
Understanding the difference in probe properties holds the key to absolute quantification of DNA microarrays. So far, Langmuir-like models have failed to link sequence-specific properties to hybridization signals in the presence of a complex hybridization background. Data from washing experiments indicate that the post-hybridization washing has no major effect on the specifically bound targets, which give the final signals. Thus, the amount of specific targets bound to probes is likely determined before washing, by the competition against nonspecific binding. Our competitive hybridization model is a viable alternative to Langmuir-like models.
Publication Title
Physical Biology
Volume
7
Issue
4
Recommended Citation
Li, S.,
Pozhitkov, A.,
Brouwer, M.
(2010). Linking Probe Thermodynamics to Microarray Quantification. Physical Biology, 7(4).
Available at: https://aquila.usm.edu/fac_pubs/834