Extra-Mitochondrial Localization and Likely Reproductive Function of a Female-Transmitted Cytochrome C Oxidase Subunit II Protein
Document Type
Article
Publication Date
6-1-2009
Department
Biological Sciences
School
Biological, Environmental, and Earth Sciences
Abstract
Our previous study documented a reproductive function for the male‐transmitted mitochondrial DNA (mtDNA)‐encoded cytochrome c oxidase subunit II (MCOX2) protein in a unionoid bivalve. Here, immunoblotting, immunohistochemistry and immunoelectron microscopy analyses demonstrate that the female‐transmitted protein (FCOX2) is: (i) expressed in both male and female gonads; (ii) maximally expressed in ovaries just prior to the time of the annual fertilization event; (iii) displayed in the cytoplasm and more strongly in the plasma membrane (microvilli), vitelline matrix and vitelline envelope of mature ovarian eggs; and (iv) strongly localized to the vitelline matrix of some eggs just prior to fertilization. These findings represent evidence for the extra‐mitochondrial localization of an mtDNA‐encoded gene product and are consistent with multifunctionality for FCOX2 in eggs.
Publication Title
Development Growth and Differentiation
Volume
51
Issue
5
First Page
511
Last Page
519
Recommended Citation
Chakrabarti, R.,
Shepardson, S.,
Karmaker, M.,
Trdan, R. J.,
Walker, J. M.,
Shandilya, R.,
Stewart, D. T.,
Vijayaraghavan, S.,
Hoeh, W.
(2009). Extra-Mitochondrial Localization and Likely Reproductive Function of a Female-Transmitted Cytochrome C Oxidase Subunit II Protein. Development Growth and Differentiation, 51(5), 511-519.
Available at: https://aquila.usm.edu/fac_pubs/8435