Isolation and Characterization of Microsatellite Markers in Fraser Fir (Abies fraseri)
Document Type
Article
Publication Date
3-1-2006
Department
Biological Sciences
School
Biological, Environmental, and Earth Sciences
Abstract
We describe the isolation and characterization of 14 microsatellite loci from Fraser fir (Abies fraseri). These markers originated from cloned inserts enriched for DNA sequences containing tandem di‐ and tri‐nucleotide repeats. In total, 36 clones were selected, sequenced and evaluated. Polymerase chain reaction (PCR) primers for 14 of these sequences consistently produced simple PCR profiles and were found to be polymorphic among 13 Fraser fir samples. In addition, more than half of these loci were found to amplify a wide range of samples from several Abies taxa.
Publication Title
Molecular Ecology Notes
Volume
6
Issue
1
First Page
65
Last Page
68
Recommended Citation
Josserand, S.,
Potter, K.,
Johnson, G.,
Bowen, J.,
Frampton, J.,
Nelson, C.
(2006). Isolation and Characterization of Microsatellite Markers in Fraser Fir (Abies fraseri). Molecular Ecology Notes, 6(1), 65-68.
Available at: https://aquila.usm.edu/fac_pubs/8563