NMR Study of Chloroplast Water in Acer platanoides: Water Exchange at Membrane-Bound Sites and Across the Chloroplast Envelope Membrane

Document Type

Article

Publication Date

3-1-2000

Department

Biological Sciences

School

Biological, Environmental, and Earth Sciences

Abstract

Nuclear magnetic resonance (NMR) spin relaxation techniques were used to measure the rate of water exchange across the chloroplast envelope membrane and to detect exchange of water‐hydrogens between the bound and fluid states in Acer platanoides shade leaves. Results indicate that individual water molecules resided within a chloroplast for 320 ± 65 ms, on average, before exchanging through the envelope, and that exchangable bound hydrogens are at least six times more abundant in the chloroplast than in the nonchloroplast compartments.

Publication Title

Plant Biology

Volume

2

Issue

2

First Page

204

Last Page

207

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