Isolation and Characterization of Polymorphic Microsatellite loci for the Gopher Tortoise (Gopherus polyphemus) and Cross-Amplification in other Species of Gopherus
Document Type
Article
Publication Date
3-1-2013
Department
Biological Sciences
School
Biological, Environmental, and Earth Sciences
Abstract
All members of the tortoise genus Gopherus have some degree of conservation concern. We report on the development of thirty-two new microsatellite loci for gopher tortoise, which were tested on 31 individuals from Ft. Benning, Georgia, USA. These loci possessed an average number of 5.09 alleles, an average observed heterozygosity of 0.574 and an average expected heterozygosity of 0.589. We also tested these loci in three congeners: Gopherus morafkai, Gopherus flavomarginatus and Gopherus berlandieri. The new microsatellite loci should prove useful in conservation efforts of Gopherus polyphemus and other Gopherus species.
Publication Title
Conservation Genetics Resources
Volume
5
Issue
1
First Page
255
Last Page
259
Recommended Citation
Kreiser, B. R.,
Ennen, J. R.,
Gaillard, D. L.,
Qualls, C. P.
(2013). Isolation and Characterization of Polymorphic Microsatellite loci for the Gopher Tortoise (Gopherus polyphemus) and Cross-Amplification in other Species of Gopherus. Conservation Genetics Resources, 5(1), 255-259.
Available at: https://aquila.usm.edu/fac_pubs/8806