Synthesis of Symmetrical Thiol-Adenosine conjugate and 5 ' thiol-RNA Preparation by Efficient One-Step Transcription
Document Type
Article
Publication Date
11-1-2010
Department
Chemistry and Biochemistry
School
Mathematics and Natural Sciences
Abstract
A symmetrical transcription initiator containing two adenosines and conjugated thiol functionality (ThioAMP dimer) is chemically synthesized. Transcription in the presence of ThioAMP dimer under the T7 Φ2.5 promoter yields 5′ thiol-labeled RNA (5′ HS-RNA) with up to 90% labeling efficiency, depending on the concentration ratio of ThioAMP dimer to ATP. The resulting 5′ HS-RNA may be used directly or after thiopropyl Separose 6B affinity column purification. Biotinylation of 5′ HS-RNA and formation of gold nanoparticle-RNA nanoplexes are demonstrated.
Publication Title
Bioorganic and Medicinal Chemistry Letters
Volume
20
Issue
21
First Page
6254
Last Page
6257
Recommended Citation
Huang, F.,
Shi, Y.
(2010). Synthesis of Symmetrical Thiol-Adenosine conjugate and 5 ' thiol-RNA Preparation by Efficient One-Step Transcription. Bioorganic and Medicinal Chemistry Letters, 20(21), 6254-6257.
Available at: https://aquila.usm.edu/fac_pubs/8901