Date of Award

Spring 2026

Degree Type

Honors College Thesis

Academic Program

Biological Sciences BS

Department

Biological Sciences

First Advisor

Dr. Nikki Camlin

Advisor Department

Biological Sciences

Abstract

PP1β is an understudied protein phosphatase that affects the cell’s ability to reproduce. It has been known to be an important part of the cell cycle. Mutations within PP1β have been found to affect that process and influence the development of cancer and disease. In this thesis, different PP1β cancer and disease associated polymorphisms are investigated through the use of humanized yeast. PP1β yeast variants are tested by using Live/Dead cell assay and western blot techniques. Live/Dead analysis was conducted on different strains grown at permissible temperature (room temperature) and non-permissible temperature (37°C) over the course of 6 or 48 hours. Results show a significant difference in the abundance of living cells compared to dead cells between the two temperature growths. Western blotting was conducted on the same variants of yeast the Live/Dead analysis was used on. Results showed a significant difference in growth of the 37°C yeast when comparing the mutant and wild-type strains. In conclusion, PP1β variants have an effect on the ability of cells to grow.

Comments

Blanket Permission for Future Readers: Uncertain, please contact me with future requests at the following NON-USM email address: kgmart04@outlook.com

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