Date of Award
Spring 5-2016
Degree Type
Honors College Thesis
Department
Biological Sciences
First Advisor
Alex S. Flynt
Advisor Department
Biological Sciences
Abstract
MicroRNAs are approximately 22-nucleotide long RNA molecules that function through decay and translational repression of messenger RNA. The microRNA let-7 is found to play a role in maintaining the fate of differentiated cells in humans. Thus, expression level of this microRNA is a reliable biomarker of tumor cell phenotype. However, there are significant limitations in the current profiling techniques of microRNA. The current methods like northern blotting, microarrays, RT-PCR, or using locked nucleic acid (LNA) for in-situ hybridization are either laborious, semi-quantitative, or expensive. In this research we try to address this issue by developing a fast, specific, and inexpensive method. This method utilizes the intrinsic properties of the conducting polymer polyaniline (PANI) as a sensing platform to detect specific target miRNAs.
Copyright
Copyright for this thesis is owned by the author. It may be freely accessed by all users. However, any reuse or reproduction not covered by the exceptions of the Fair Use or Educational Use clauses of U.S. Copyright Law or without permission of the copyright holder may be a violation of federal law. Contact the administrator if you have additional questions.
Recommended Citation
Gloria, Jared N., "Using Intrinsic Properties of Polyaniline to Sense Expression of the microRNA Let-7" (2016). Honors Theses. 409.
https://aquila.usm.edu/honors_theses/409