Production of Antibodies for Specific Detection of Dengue Virus-2 By Surface Enhanced Raman Spectroscopy
Date of Award
Honors College Thesis
Dengue Virus (DENV) is a flavivirus spread by mosquitoes that are endemic in tropic and subtropic climates. It is the causative pathogen of Dengue Fever, Dengue Hemorrhagic Fever, and Dengue Shock Syndrome. Besides mosquito transmission, DENV can also be transmitted through blood transfusion. Due to the high expense and expertise needed to run tests like ELISA and Polymerase Chain Reaction (PCR) based-assays for DENV detection, a rapid, sensitive and cost effective gold nanoparticles mediated surface-enhanced Raman spectroscopy (SERS) assay has been developed as an alternative assay to detect DENV. In this project, I successfully produced a monoclonal antibody that specifically binds to DENV-2 from the HB46-ATCC cell line. Using HiTrap™ Protein G HP columns, the antibodies were purified and concentrated by SpinX centrifuge filters. Bradford assay was used to measure antibody concentration, SDS-PAGE was used to detect possible protein contamination, and immunostaining confirmed antibody specificity for DENV 2. The SERS results show that this antibody can be used for specifically detection of DENV-2.
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Thompson, E. Ashley, "Production of Antibodies for Specific Detection of Dengue Virus-2 By Surface Enhanced Raman Spectroscopy" (2017). Honors Theses. 474.